WATERS 186001346 Atlantis Column DC18 4.6 250 214800-R1 B7

( Brand: Waters ), ( Manufacturer Part Number: 186001346 ), ( Type: Column ), ( Country Of Origin: Ireland )

Review WATERS Atlantis Column 100 4.6mm 250 #214800-r1 B7

The **Waters 186001346 Atlantis dC18 4.6 x 250 mm, 5 m, 100 (214800-R1, B7)** is a high-performance reverse-phase analytical column designed for demanding separations in liquid chromatography (LC), particularly in applications requiring exceptional resolution, sensitivity, and robustness. Engineered with Waters proprietary **Atlantis dC18** stationary phase, this column features a **fully porous, core-shell particle technology** that combines the efficiency of sub-2 m particle size with the mechanical stability of larger particles, delivering superior peak shapes, faster equilibration times, and enhanced column lifetime. The **4.6 mm internal diameter** and **250 mm length** provide an optimal balance between sample capacity and chromatographic efficiency, making it ideal for a wide range of analytical and preparative separations, including small molecule analysis, pharmaceutical compound profiling, and bioanalytical studies. The **5 m particle size** ensures a robust compromise between backpressure and resolution, while the **100 pore size** facilitates efficient retention and separation of mid-to-high molecular weight analytes, including peptides, proteins, and complex small molecules. The **dC18 chemistry** offers a balanced hydrophobicity, reducing tailing for basic and polar compounds while maintaining strong retention for hydrophobic analytes a critical advantage in applications involving ionizable or polar drugs. This column is compatible with a broad spectrum of mobile phase compositions, including aqueous-organic gradients, making it versatile for both isocratic and gradient elution techniques. Additionally, the **B7 (blue) end cap** indicates a specialized surface modification that further enhances peak symmetry and reduces non-specific binding, improving reproducibility and sensitivity in challenging separations. Whether used in **UPLC, HPLC, or LC-MS applications**, this column excels in delivering high-resolution separations with minimal peak distortion, making it a reliable choice for laboratories demanding precision, consistency, and long-term performance.

### **Pros and Cons of buying a Waters 18600-1346 Atlantis dC18 Column (4.6 x 250 mm, 214800-R1, B7)**

1. **High Performance for Reversed-Phase HPLC/UPLC** The Atlantis dC18 column is designed for high-resolution separations, making it suitable for complex biological, pharmaceutical, and environmental samples. Its unique surface chemistry provides excellent peak shape and efficiency, reducing tailing and improving sensitivity.

2. **Wide Compatibility with Mobile Phases** The B7 (balanced) end-capping and dC18 chemistry allow for flexibility in gradient and isocratic elution methods, accommodating a broad range of solvents (e.g., water-acetonitrile, water-methanol) without excessive backpressure or peak distortion.

3. **Durability and Longevity** Waters Atlantis columns are known for their robust construction, with a 4.6 mm internal diameter and 250 mm length providing a good balance between sample load capacity and resolution. Properly maintained, this column can last for thousands of injections, reducing long-term replacement costs.

4. **Consistent Batch-to-Batch Performance** Waters maintains strict quality control, ensuring reproducibility across different column batches. This is critical for laboratories that rely on consistent analytical results for regulatory or clinical applications.

- **Pharmaceutical analysis** (e.g., drug purity, chiral separations with proper chiral additives).

- **Biological samples** (e.g., peptides, proteins, metabolites).

- **Environmental testing** (e.g., pesticides, PCBs, endocrine disruptors).

- **Food and beverage analysis** (e.g., contaminants, additives).

6. **Backed by Waters Technical Support** As a reputable brand, Waters offers comprehensive documentation, troubleshooting guides, and customer support, which can be invaluable for optimizing method development and troubleshooting issues.

7. **Lower Backpressure Compared to Smaller Particles** While not as fast as sub-2 m columns, the 3 m particle size (implied by the model number) strikes a balance between speed and efficiency, reducing the need for ultra-high-pressure UPLC systems for many applications.

---

1. **Higher Upfront Cost** Compared to generic or lower-end columns from other manufacturers, the Atlantis dC18 is a premium product. For laboratories with tight budgets, this may be a significant investment, especially if multiple columns are needed.

2. **Not Ideal for Ultra-High-Speed Separations** While efficient, the 3 m particle size and 4.6 mm ID are not optimized for the fastest possible separations (which would require sub-2 m particles and narrower columns). If speed is critical, a smaller particle size (e.g., 1.7 m) or narrower bore (e.g., 2.1 mm) may be preferable.

3. **Requires Proper Maintenance** Like all HPLC columns, the Atlantis dC18 benefits from regular cleaning (e.g., with isopropanol or dedicated cleaning protocols) and proper storage (e.g., in a sealed vial with a guard column). Neglecting maintenance can lead to premature degradation or contamination.

4. **Potential for Column Bleeding Over Time** Some reversed-phase columns, including dC18 variants, may exhibit slight bleeding of stationary phase over extended use, particularly under harsh conditions (e.g., high organic content, high temperatures). This can affect baseline stability in sensitive applications.

5. **Guard Column Replacement Costs** While not specific to this column, Atlantis dC18 columns typically require a guard column (e.g., Waters Atlantis dC18 Guard-Pak) to protect the main column from particulates and contaminants. Replacing guard columns adds to the long-term operational cost.

6. **Overkill for Simple Applications** For very straightforward analyses (e.g., basic drug screening with minimal sample complexity), a more affordable generic C18 column might suffice. The Atlantis dC18 s advantages are more pronounced in challenging separations.

---

### **Conclusion**

The **Waters 18600-1346 Atlantis dC18 column (4.6 x 250 mm)** is a high-quality, versatile choice for laboratories requiring reliable, high-resolution separations across a wide range of applications. Its balanced chemistry, durability, and compatibility with diverse mobile phases make it a strong investment for pharmaceutical, biological, and environmental analyses. However, its premium price and the need for proper maintenance may not justify its use in all settings, particularly for simple or high-throughput applications where cost efficiency is critical.

### **Recommendation**

- You need a **high-performance, long-lasting column** for complex or critical analyses (e.g., drug development, clinical diagnostics, environmental monitoring).

- Your laboratory prioritizes **reproducibility and technical support** from a trusted manufacturer.

- You are working with **samples that require sharp peaks and minimal tailing** (e.g., peptides, chiral compounds).

- You can afford the **upfront cost** and are willing to invest in proper maintenance (guard columns, cleaning protocols).

- Your budget is limited, and you can achieve similar results with a **lower-cost generic C18 column** (e.g., Phenomenex Luna C18, Agilent Zorbax Eclipse Plus).

- You require **ultra-fast separations**, in which case a **sub-2 m particle size column (e.g., Waters Acquity UPLC BEH C18)** or a **narrower bore (2.1 mm)** would be more appropriate.

- Your applications are **simple and high-throughput**, where a **disposable or lower-resolution column** might suffice.

For most **serious analytical laboratories**, the Atlantis dC18 remains a **strong, reliable choice** just ensure it aligns with your specific needs and budget constraints.

Details:

#214800-R1 B7. Waters 186001346. All return shipping charges must be prepaid by the customer. #214801-R1 B7 # 815.

Atlantis dC18 Column, 1/5 m, 4.6mm X 250.

part #: 186001346 prices

• $149.99-$699.00

specifications automationjack:

• brand: Waters
• mpn: 186001346
• type: Column
• country of origin: Ireland

general automationjack:

• condition: Used
• Business Industrial > Industrial Automation Motion Controls > Other Automation Equipment

seller automationjack:

• rating: 100.0%
• # reviews: 1324
• city: Moreno Valley, California

shipping automationjack:

• type: Calculated
• transit: 4-10 Days
• class: Standard
• service: UPS Ground, FedEx Ground / FedEx Home Delivery
• carrier: UPS, FedEx
• cost: $32.41

returns automationjack:

• policy: No Returns

offer automationjack:

• sold: 0
• availability: In Stock
• quantity: 9
• best offers: True
• options: Ship-to-home
• started: March 3, 2026

payments automationjack:

• wallet: Google-pay
• other
• credit card: Visa

Waters 186001346 Is Similar To:

• Waters 186001265 Pah C18 5um 4.6 X 250mm Column P n S n T21421a 11 (49.3% similar) Free shipping!, Waters pah C18 5UM 4.6 x 250MM column made in Germany, p/n: 186001265 s/n: T21421A 11...(October 5th, 2024)
• Waters 186003127 Xbridge Beh C18 130a 2.5 M 4.6mm X 75mm Column P n 186003124 (41.6% similar) Recommended applications:: pharmaceutical drug development, environmental food testing, complex mixture unknown sample analysis, high-performance reversed-phase separations. Waters bridge Beth C18 2.5 m 4.6MM x 75MM plc column: retail price: $600 for a great deal. Or make an offer. Message me ...(February 15th, 2025)
• Waters 186006747 Xselect Hss Pfp 2.5 M 4.6 X 150 Mm Column Xp (49.6% similar) Shiatsu prominence and next series. The 2.5 m extended performance PX particle size, packed in ultra-low dispersion hardware, offers robust performance, high throughput, and seamless scalability to ultra liquid chromatography UPC systems. Trusted brand: manufactured by Waters corporation, a global ...(June 16th, 2025)
• Waters Wat054215 C18 5 M 4.6x250mm Column (44.2% similar) From a pharmaceutical QC laboratory. 8062, used column as shown...(December 6th, 2025)
• Waters 186003900 Xbridge Prep C18 Column 10 M Obd 50 Mm X 250 (60.2% similar) I purchased this secondhand column around 2012 , it has been tested and never installed or used since then...(February 27th, 2026)
• Waters 186003613 Xbridge Beh300 C18 3.5 M 4.6 X 150mm Hplc Column (49.6% similar) Used Waters bridge 186003613 BEH300 C18 3.5 m 4.6 x 150MM plc column, lot CON4/4BX14WATPARTS...(March 18th, 2026)